Finally, we took the intersection of the genes obtained from the LASSO regression and univariate Cox regression model, and obtained 8-FRGs significantly associated with LC prognosis, including VDAC2, HSF1, ACSL3, PANX1, FADS2, PHKG2, GLS2 and CDKN1A (Fig. 3D). This evidence concerns the gene PHKG2 and laryngotracheoesophageal cleft.