To the best of the author’s knowledge, this is the 1st time that GCF Gal-1 and Gal-3 were investigated together in periodontal disease before and after non-surgical periodontal treatment in an attempt to validate their value to be utilized as biomarkers for periodontal disease diagnosis, showing their effect on disease progression and monitoring treatment effectiveness. This evidence concerns the gene LGALS1 and periodontal disorder.