TP53 and cancer: These included two previously diagnosed cancer patients who were referred for genetic tested using targeted TP53 p.Arg181Cys PCR, as a result of an incidental finding of the variant in two pediatric non-cancer probands (ages 4 and 18), who underwent WES due to developmental delay, and consequent cascade testing of their relatives (based on ACMG recommendations for reporting of secondary findings from WES [23]).