Furthermore, SARS-CoV-2 D614G[Omicron spike] infection resulted in a significantly increased expression of ISGs Mx1, Oas1, and Viperin (Figure 4E) as well as Cxcl10 (Supplementary Figure S4), while pro-inflammatory mediators Cxcl1, Ccl2, and Il6 were not significantly altered in any group compared to the uninfected mice (Supplementary Figure S4). This evidence concerns the gene CCL2 and infection.