RNA‐seq analysis revealed that, compared to the control group, LV‐EHF overexpression led to significant upregulation of 154 genes in HuCCT1 cells, whereas the AKT/YAP/EHF group showed significant upregulation of 1358 genes in mouse CCA tissues compared to the AKT/YAP/PT3 group (Figure 4A). This evidence concerns the gene EHF and cholangiocarcinoma.