To further investigate the biological behavior of EHF in CCA and its role in tumor development, we stably overexpressed EHF in HuCCT1 and HCCC9810 cell lines with low expression of EHF using a lentivirus and stably knocked down EHF in QBC939 and TFK‐1 cell lines with high expression of EHF using short hairpin RNA (shRNA), with SH‐EHF‐1 showing better knockdown efficiency (Figure 2A,B). Here, EHF is linked to cholangiocarcinoma.