To investigate the mechanism by which cytokines or chemokines are involved in EHF‐mediated CCA occurrence, we used a cytokine array to measure the secretion of cytokines from HuCCT1 cells overexpressing EHF (HuCCT1 LV‐EHF) compared to the control group (HuCCT1 LV‐CON) (Figure 5B). This evidence concerns the gene EHF and cholangiocarcinoma.