Since the secretion of pro-inflammatory cytokine (IL-6) and fibrosis related proteins (TGF-β and P3NP) showed a significant increase in a time-dependent manner, which indicates the progression of the disease to NASH, the expression levels for inflammatory (e.g, IL-6, TNF-α) and fibrosis-related genes (e.g, TGF-β, COL1A1) on days 3 and 8 were compared. The gene discussed is TNF; the disease is metabolic dysfunction-associated steatohepatitis.