TGFB1 and Hepatic fibrosis: Considering the secretome of microtissues for some key player proteins involved in NAFLD, we demonstrated that the production of all evaluated markers, i.e., IL-6, TGF-β, and P3NP (as a marker for liver fibrosis score in clinical assessment (Chen et al., 2022[9]) was considerably higher in NMT in comparison to that of LMT (Figure 3d(Fig. 3)).