The significant upregulation of IL-6 and TNF-α on days 3 and 8, and also TGF-β and COL1A1 on day 8 indicate that NMT reflects the NASH state on day 3 as well as the progression of the disease to the fibrosis stage on day 8 (Figure 4d(Fig. 4)). This evidence concerns the gene TNF and metabolic dysfunction-associated steatohepatitis.