Three differentially methylated sites in youth-onset T2D were also associated with exposure to intrauterine maternal diabetes in a dose dependent manner, all in PFKFB3. These sites showed decreased DNAm in youth of mothers with T2D which was even lower in youth who had T2D themselves, suggesting that in utero exposure to diabetes might change the DNAm profile for those sites which are then further altered if the youth develop T2D. The gene discussed is PFKFB3; the disease is diabetes mellitus.