Thirty CNVs of 82 cases [19 PCNVs, 6 benign (B) CNVs, 2 like benign (LB) CNVs, and 3 variants of uncertain significance (VOUS)] were revealed by CNV-seq, including several syndromes such as Down's syndrome (n = 4), Edward's syndrome (n = 2), 22q11.2 microdeletion syndrome (n = 1), 1p36 microdeletion syndrome (n = 1), Wolf-Hirschhorn syndrome (n = 1), and recurrent Simpson-Golabi-Behmel syndrome type 1 (SGBS1) (n = 2) (Table 4), plus extra CHD anomalies indicating related syndromes should be considered by clinicians once facing multiple fetal structural abnormalities. Here, GPC3 is linked to Down syndrome.