In the open field test, compared to the control group, the A2AR antagonist SCH58261 reduced the locomotion and increased the anxiety level, while in contrast, the A1R antagonist DPCPX did not affect locomotion and anxiety (One-way ANOVA, locomotion, F(2, 26) = 4.893, P = 0.016, Tukey’s post hoc test, P16p+VEH+40Hz-14d vs. 16p+SCH+40Hz-14d = 0.038, P16p+VEH+40Hz-14d vs. 16p+DPCPX+40Hz-14d = 0.973; anxiety, F(2, 26) = 4.824, P = 0.017, Tukey’s post hoc test, P16p+VEH+40Hz-14d vs. 16p+SCH+40Hz-14d = 0.013, P16p+VEH+40Hz-14d vs. 16p+DPCPX+40Hz-14d = 0.208, Fig. 5a–c). This evidence concerns the gene ADORA2A and Anxiety.