We chose CASC19 and LINC00460 based on several criteria: (i) their high expression levels in CRC tissues and cell lines; and (ii) the feasibility of designing CRISPRi sgRNAs targeting these genes without impacting the expression of neighbouring genes (i.e. transcriptional start site, TSS, > 10 Kb from the nearest protein coding gene). The gene discussed is CASC19; the disease is colorectal carcinoma.