After validation of STZ and Aβ1–42 induced Alzheimer’s disease model, we then checked the level of Pirh2 through immunoblotting in both N2A neuroblastoma cells and Sprague-Dawley (SD) rat brain regions (cortex and hippocampus) in both control and STZ or Aβ1–42 induced AD model. This evidence concerns the gene RCHY1 and Alzheimer disease.