High mutation of CT26 KO cells induced activation of large immune cells in mice, aggregating around CT26 KO tumors and releasing large of inflammatory factors such as perforin, granzyme B, IFN-γ and TNF-α, which induced the activation of PANopotosis effector molecules N-GSDMD, N-GSDME, cleaved-CASP3, cleaved-CASP8, and p-MLKL that inhibited CT26 KO tumor growth, while CT26 (Ctrl) had low immunogenicity and induced few activated immune cells and inflammatory factors, which could not inhibit tumor growth. Here, MLKL is linked to neoplasm.