In the tumor microenvironment formed by CT26 (Ctrl), immune effector molecules perforin, granzyme B, IFN-γ, and TNF-α were rarely expressed, and the PANoptotic molecules N-GSDMD, N-GSDME, cleaved-CASP3, cleaved-CASP8, and p-MLKL were also rarely expressed; comparison of CT26 (Ctrl) tumor microenvironment, CT26 KO1, CT26 KO2 tumor microenvironment highly expressed immune effector molecules perforin, granzyme B, IFN-γ, TNF-α and induced more expression of PANoptotic molecules N-GSDMD, N-GSDME, cleaved-CASP3, cleaved-CASP8, and p-MLKL, leading to significant differences in tumor volume. This evidence concerns the gene MLKL and neoplasm.