At the same time, we found that CT26 and CMT93 VT also exhibited natural hyperactivation of GSDMD, GSDME, and p-MLKL, because they were constructed by cas9-expressing lentiviral vector, they could induce natural hyperactivation of GSDMD, GSDME, and p-MLKL through viral infection. Here, GSDMD is linked to viral infectious disease.