The ATP release result showed IFN-γ induced little death in SW480 and HCT116, but much in DLD1; TNF-α could induce more cell death than IFN-γ in SW480 and HCT116, but little death in DLD1 (Fig. 2a); the ATP release and PI staining results showed co-treatment of IFN-γ and TNF-α induced most cell death in DLD1, HCT116, SW480, and HT29, the number of death cells in DLD1 and HCT116 was more than that in SW480 and HT29, and DLD1, HCT116 are dMMR cancer cell lines, SW480 and HT29 are pMMR cancer cell lines. This evidence concerns the gene IFNG and cancer.