HFD feeding and obesity have been associated with gut barrier disruption, increased lipopolysaccharide translocation and metabolic endotoxaemia.1 Supplementing 2’FL to HFD has shown protective effects on markers of the gut barrier, but the mechanisms were not explored.20 21 In this study 2’FL supplementation led to higher expression of antimicrobial peptides Lyz1 and Reg3g, and proglucagon, the precursor of GLP-1 and GLP-2, involved in improved gut barrier function, in specific sites of the gastrointestinal tract. This evidence concerns the gene REG3G and Obesity.