Taken together, these results, obtained using two different and complementary techniques, one evaluating protein-protein interaction in living cells (NanoBiT) and the other analysing the kinetic of interaction with purified proteins in vitro (SPR), suggest that the interaction between NF242 and TDP-43 could have a physiological effect in vivo using ALS animal models. This evidence concerns the gene TARDBP and amyotrophic lateral sclerosis.