The specific upregulationof FSP1 and CD34 within the contextof the rigid L-PIC gel matrix suggests a potential sensitivityof these genes to ECM stiffness, although further validation is desired.To sum up, the TempEasy coculture systems reproducekey hallmarks of the initial stages of the angiogenic transition inprostate cancer, a phenomenon challenging to discern due to its occurrencebefore the definitive clinical diagnosis. The gene discussed is S100A4; the disease is cancer.