In this study, we conducted in silico analysis to determine the pattern of CYB561 expression across different prostate tumor subtypes, and characterized the basal and androgen-dependent regulation of CYB561 expression in in vitro cell models representative of normal prostate epithelium, androgen-dependent metastatic disease, hormone-indolent CRPC, and NEPC. This evidence concerns the gene CYB561 and prostate neoplasm.