MB725 was found to exhibit a strong and selective reduction in viability in the p53-Y220C cancer cell lines BXPC-3, HUH-7, and NUGC3 at concentrations below 40 μmol/L, while it maintained a relatively low toxicity in the same concentration range in the p53-R273C mutant cell line SW1088 and the p53 WT cell lines WI38 (normal fibroblast cell line) and NUGC4[63]. Here, TP53 is linked to cancer.