Age correlated positively with the non-pregnancy (r=0.221, p<0.001;***), FSH (r= 0.267, p<0.001;***), duration of infertility (r=0.122, P=0.028), the total number of transferred embryos (r=0.346, p<0.001;***), LH (r=0.130, p=0.019), and with the number of unsuccessful IUI (r=0.117, p=0.036) and negatively with singleton pregnancy (r= - 0.219, p<0.001;***) rate, AMH (r=- 0.243, p<0.001;***), and number of follicles (r=–0.286, p<0.001;***). The gene discussed is PLOD1; the disease is Infertility.