Biomarkers of 8 hub genes and infiltration analysis of 28 immune cells suggested that in VD gene concentration, central memory CD4 T cells are significantly negatively correlated with all hub genes (P < 0.05), while MDSC and NK T cells are positively correlated with all hub genes (P < 0.05), indicating that hub genes have a good level of infiltration with immune cells in VD. This evidence concerns the gene CD4 and sexually transmitted disease.