MYLK and colorectal cancer: In our experiment, we extracted RNA and protein from 6 colorectal cancer cell lines and confirmed the different expression levels of MLCK1 in different colorectal cancer cells by western blot and RT-PCR, and results suggested that the expression levels of MLCK1 were higher in CACO2 and HT29 cells and lower in LOVO and SW480(Fig. 2a–c).