For the cell line HT29 with high expression of MLCK1, the sensitivity of HT29 colon cancer cells to 5-Fu was significantly increased after knocking down the expression of MLCK1, and the flow cytology results after 5-Fu adding suggested higher cell mortality, p < 0.05, statistically significant difference (Fig. 3f, g). Here, MYLK is linked to colonic neoplasm.