After plasmid transfection of two screened low-expressing MLCK1 cell lines (LOVO and SW480) overexpressing MLCK1, cell death in control and dosing groups was detected using flow apoptosis, and the results suggested that for LOVO and SW480 cell lines, colon cancer cells were significantly less sensitive and more resistant to 5-Fu after overexpressing MLCK1 (Fig. 3a), the p < 0.05, statistically significant difference (Fig. 3b, c). Here, MYLK is linked to malignant colon neoplasm.