To independently validate gene expression changes in the thymus, 3 DEGs with FC > 1.8 and detailed/functional annotation (TF, GATA3, FCLR2 and FLT4) were selected for further validation by qPCR using RNA samples from 16 MG patients (8 TAMG and 8 TFH-MG). The gene discussed is FLT4; the disease is myasthenia gravis.