Previously we searched for methylated tumor suppressor genes (TSGs) candidates in digestive cancers through epigenomic (CpG methylome) study and expression profiling of a paired colon cancer cell line (HCT116 and HCT116-DKO with double knock-out of DNMT1 and DNMT3B [25, 26]), and found a significant signal enrichment of CpG methylation at the FOXE1 promoter in HCT116, but not in DKO, suggesting that FOXE1 is methylated target in colorectal cancer. The gene discussed is FOXE1; the disease is malignant colon neoplasm.