Here, we first established a non-metabolic PCOS mouse model to exclude the effect of metabolic factors and further clarified that IL-22 relies on STAT3 to ameliorate follicle development and ovulation of non-metabolic PCOS mice by constructing granulosa cell-specific STAT3 knockout (Fshrcre+Stat3f/f) mice. This evidence concerns the gene STAT3 and polycystic ovary syndrome.