This was also evident 48 h post-infection with a mean increase in the relative expression fold change of 10.02 ± 0.95 (p-value < 0.001) for CYP27B1 (Figure 2D), and 3.37 ± 0.71 (p-value < 0.05) for CAMP (Figure 2F) compared to the control, and the MAP-infected only groups. This evidence concerns the gene CYP27B1 and infection.