We tested the ability of the MO-I-1151 ASPH inhibitor alone or in combination with a recently described SULF2 inhibitor HfFucCS [34] with regard to the invasion of HNSCC tumor cells in a spheroid co-culture model with a primary HNSCC cancer-associated fibroblast (CAF 61137). Here, ASPH is linked to head and neck squamous cell carcinoma.