Using NTA-associated fluorescence on platelet-free plasma from each participant, we found that all the healthy (n = 10), CHF (n = 10), and DHF (n = 10) participants had detectable levels of EV-hERG1 and EV-Hsp47 in their PFP samples, with similar distributions in the sizes of the sEVs (Figure 6A). The gene discussed is PRF1; the disease is congestive heart failure.