Previous studies showed that the neuroprotective benefits of 17β-estradiol (E2) ameliorated cholinergic deficit, elevated the expression levels of choline acetyltransferase and 5-hydroxytryptamine receptor 2A, and lowered the expression of GFAP in a rat model of Alzheimer’s disease induced by OVX [77]. Here, CHAT is linked to early-onset autosomal dominant Alzheimer disease.