Considering the amount of MMP-2 expressed by the selected primary human cell line of GBM used for the in vitro tests after 72 h of culture, 4.57 ng/mL of this protease was incubated with the RGDS-HA hydrogel to demonstrate its ability to cleave the amide bond between RGDS and HA. This evidence concerns the gene MMP2 and glioblastoma.