In line with this, the gene expression involved in bile acids synthesis and excretion, including Cyp7a1, Abcg5 and Abcg8, was markedly upregulated through increased endogenously synthesized n-3PUFAs in a high fat diet-induced MASLD murine model [28], suggesting that n-3PUFAs may facilitate cholesterol excretion. Here, ABCG5 is linked to metabolic dysfunction-associated steatotic liver disease.