RNA sequencing (RNA-seq) and confirmatory expression studies in our laboratories demonstrated robust expression of Forkhead box I1 (Foxi1) transcription factor and its downstream targets, including apical H+-ATPase transmembrane (V0) and catalytic (V1) components, in the cyst epithelia of Tsc1 as well as Tsc2 knockout mice, but not in mice with the Pkd1 gene mutation [9]. Here, PKD1 is linked to cyst.