To explore whether ERK signaling participated in kaempferol’s regulation of VEGF, it transfected ovarian cancer cells with ERK1 plasmid and noticed that kaempferol’s inhibition on VEGF transcriptional activation, as reflected by VEGF reporter luminescence, was stopped by forced ERK1 expression in a concentration-dependent way, and a significance was attained in A2780/CP70 cells. The gene discussed is VEGFA; the disease is ovarian carcinoma.