DEFB1 and neoplasm: Our study determined the TB grade according to the recommendations of the International Tumor Budding Consensus Conference (ITBCC) in 2016, where the bud count was divided into three-tier grades: low-grade Bd1 (0–4 buds), intermediate-grade Bd2 (5–9 buds), and high-grade Bd3 (≥10 buds) [28].