The following samples, collected prior to Atezo/Bev treatment, were analyzed for their association with the initial treatment response: (1) expression of 14 genes associated with cancer progression in CTC RNA, (2) number of CTCs, (3) tumor differentiation and PD-L1 expression in liver tumor biopsy tissues, and (4) clinical features (Figure 1D). This evidence concerns the gene CD274 and neoplasm.