Since BRAF/MEK inhibitors induce both apoptosis, as well as a cell cycle arrest, in melanoma cells, we used the cell cycle analysis as an alternative readout to clarify the unresponsiveness of cell lines such as Sk-Mel-28, UACC257 and MDA-MB-435 in the viability readouts to treatment under nonadherent conditions after 24 h [52,53]. The gene discussed is BRAF; the disease is melanoma.