To evaluate changes in protein expression, select proteins identified by network analysis were used for semiquantitative immunoblotting (Figure 5A–E), including Pfn1 (Profilin-1), Acy1 (Aminoacylase-1A), and Vcl, plus met-Vcl (Vinculin, plus metavinculin) to elucidate progressive changes occurring as ACF develop into adenomas. Here, VCL is linked to adenoma.