To investigate the role of lncRNA NUTM2A-AS1 and miR-376a-3p in glioma, the expression levels of lncRNA NUTM2A-AS1 and miR-376a-3p in human glioma cell lines (U251, T98-G, A172) and glial cell lines (HEB) were measured by qRT-PCR. The gene discussed is NUTM2A; the disease is central nervous system cancer.