Malignant tumour cells were more abundant in the high-score group, whereas the low-score group exhibited greater abundances of immune cells, especially cytotoxic immune cells, such as cytotoxic (CD8+) T cells, consistent with the results of prognostic analysis and single-cell sequencing (Fig. 5C, Supplementary Fig. 4D-E and Supplementary Fig. 5G-H). The gene discussed is CD8A; the disease is neoplasm.