Also, it is reported that CCL18+ macrophages could activate NF-κB pathway in fibroblasts and induce the stemness and resistance of cancer cells [50].In this study, pseudotime analysis was performed to gain further insight into mechanisms and results indicated that MTHFD1 expression increases during monocyte-to-macrophage differentiation, suggesting its role in immunosuppressive macrophage differentiation in NSCLC. Here, MTHFD1 is linked to cancer.