Furthermore, our study indicates that methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), a key suppressor gene in choline metabolism, is correlated with an immunosuppressive microenvironment and involved in macrophage differentiation as well as endothelial cell proliferation, thus contributing to a deeper understanding of the intricate mechanisms underlying choline metabolism in the development of NSCLC. The gene discussed is MTHFD1; the disease is non-small cell lung carcinoma.