Specifically, the study uncovers the overexpression of MTHFD1 in abnormal choline metabolism was intimately associated with TAMs, shedding light on its role in immunosuppressive macrophage differentiation and endothelial cell proliferation, thus providing valuable insights into the intricate workings of choline metabolism in NSCLC pathogenesis. Here, MTHFD1 is linked to non-small cell lung carcinoma.