TWIST1 and non-small cell lung carcinoma: Consistently, the protein and mRNA levels of EMT-related transcription factors and mesenchymal markers including Twist1, ZEB1, Snail, Slug, N-cadherin, Fibronectin, and Vimentin were increased and those of the epithelial marker E-cadherin were decreased in NSCLC cells with higher PD-L1 expression (Fig. 1c−e and Supplementary Fig. S1B).