Moreover, the annotated stromal region overexpressed classical marker genes for fibroblasts or endothelial cells, such as LUM, VIM, COL1A1, and COL1A2. It is noted that the differential expression of VIM gene in comparison of stromal regions and other regions in CRC ST-seq dataset was also identified in CRC scRNA-seq dataset, in which VIM gene was one of the DEGs in malignant cells compared to non-malignant cells. This evidence concerns the gene COL1A1 and colorectal carcinoma.