Given the high expression of the LIMS1 antigen in RPE cells and the IgG dominance of anti-LIMS1 autoantibody, we treated ARPE-19 cells with purified IgG from the sera of subjects with emmetropia (emmetropia IgG), simple high myopia (simple HM IgG), or pathologic myopia (PM IgG), or with PM IgG depleted of anti-LIMS1 autoantibody (anti-LIMS1 depleted PM IgG), respectively, to examine its effects on RPE cells. This evidence concerns the gene LIMS1 and pathological myopia.