This heightened expression may potentially correlate with the heightened malignancy associated with this particular glioma subtype, as indicated by the statistical significance of P < .001 in Figure 1E and F. Western blot results of clinical samples showed that the content of CDCA3 in grade 4 gliomas was significantly higher than that in normal brain tissue and grade 2 and 3 gliomas (Fig. 1G and H), and in grade 3 gliomas, it is also higher than in normal brain tissue (P < .01, Fig. 1G and H). This evidence concerns the gene CDCA3 and central nervous system cancer.