Initially, univariate Cox regression analysis revealed that 12 genes were associated with the prognosis of breast cancer, followed by LASSO analysis to derive a prognostic signature composed of 8 genes (Figure 3B, 3C), including CERCAM, EMP1, SDC1, PRKG1, XG, TNN, WLS, and PDLIM4 (Supplementary Table 3). The gene discussed is PRKG1; the disease is breast cancer.