As expected, our study also found that TCRs of TILs with the highest average expression level of IFNG or TNFRSF9 mRNA using high-throughput RNA-seq could specifically recognize matched ATCs in each patient, suggesting that high-throughput RNA-seq provides a sensitive and efficient procedure to identify dysfunctional tumour-reactive T cells with lower levels of activation marker expression and effector molecule secretion. This evidence concerns the gene IFNG and neoplasm.