PROM1 and melanoma: Given that CD133 upregulates AREG, which has been implicated in cell proliferation via its binding to and activation of EGFR, which then activates the MAPK pathway [32], we next examined the phosphorylation and activation of EGFR as well as members of the MAPK pathway in two different melanoma cell lines (BAKP and POT) in response to Dox-induced CD133 expression.