To substantiate our above conclusions that oocyte IVA increases anxiety-like behavior and impairs spatial/fear learning/memory of offspring, expression of anxiety and learning/memory-related genes including glucocorticoid receptor (Gr), brain-derived neurotropic factor (Bdnf) and N-methyl-D-aspartate (NMDA) receptor subunit (Nr2a) were measured by RT-qPCR in hippocampi of the F1 offspring. Here, BDNF is linked to Anxiety.