To further examine these fibroblast-specific transcriptional changes in DCM, their expression using RT-qPCR was measured in an in vitro model of TGFβ-induced activation, a cytokine known to be present at high levels in DCM myocardium [14] and which was found to be upregulated in NF vs DCM bulk RNAseq (Supplementary Table S7). This evidence concerns the gene TGFB1 and familial dilated cardiomyopathy.