Afterwards, we tried to dissect the effect of ILC2 mediated by Pru p 3 allergen and their combinations with cocktail cytokines in the immunological response in LTP-AP and observed that allergen-stimulated ILC2 promote an effector response in vitro that was accompanied by an absence of Tbet+Th1- and FOXP3+Treg cell proliferation with suppression of IL-10, IL-27, and IFNγ production, confirming the lack of regulatory response in the allergic reaction (44). This evidence concerns the gene TBX21 and allergic disease.