MTUS1 and head and neck squamous cell carcinoma: To investigate whether the localization of MTUS1/ATIP1 in mitochondria and how this localization influences mitochondrial function in HNSCC, we employed the methods of Immunofluorescence and coimmunoprecipitation, Protease K assays, MitoTracker staining, seahorse assays, etc. Pyroptosis and its mechanism were examined by high-content microscopic imaging, immunofluorescence staining and western blotting and lactate dehydrogenase (LDH) release assay.