NLRP3 and periventricular leukomalacia: However, we verified that the presence or absence of Z-VAD-FMK induced high amounts of polymerized ASC and NLRP3 and enhanced FADD and GSDMD-N in PVL-induced PHG mice without shFADD, while these effects were not altered in the epithelial fractions of mice with PHG treated with shFADD, regardless of Z-VAD-FMK treatment.