To test whether PKM2 is involved in FADDosome-regulated glycolysis in PHG, we detected its downstream proteins following shFADD transfection and found that FADD knockdown by shFADD repressed LHDA upregulation in mice with PVL without affecting the levels of PKM2 isomeric forms (monomer, dimer or tetramer), Glut1 or PDK1 (Figure S4A). This evidence concerns the gene FADD and periventricular leukomalacia.